Energy preservation for skeletal muscles: Shenqi Fuzheng injection prevents tissue wasting and restores bioenergetic profiles in a mouse model of chemotherapy-induced cachexia

doi:10.1016/j.phymed.2023.155269

KMS@SIMM

	Energy preservation for skeletal muscles: Shenqi Fuzheng injection prevents tissue wasting and restores bioenergetic profiles in a mouse model of chemotherapy-induced cachexia
	Li, Wei 1,2; Zhang, Zijia 1,2; Berik, Entezar 1,3; Liu, Yawen 1,2; Pei, Wenqiang 1; Chen, Sihan 1,2; Wu, Wenyong 1,4; Wang, Zhaojun 1,2; Kong, Xinqin 1,2; Long, Huali 1,2; Lei, Min1,2 ; Wang, Jennifer Yiyang 1; Li, Zhaoxia 3; Liu, Liangfeng 5,6; Hou, Jinjun1,2 ; Wu, Wanying1,2,4 ; Guo, De -an 1,2
通讯作者	Hou, Jinjun(jinjun_hou@simm.ac.cn) ; Wu, Wanying(wanyingwu@simm.ac.cn)
	2024-03-01
发表期刊	PHYTOMEDICINE (IF:3.61[JCR-2017],3.59[5-Year])
ISSN	0944-7113
卷号	125 页码:13
摘要	Background: Energy deficiency is the characteristic of chemotherapy-induced cachexia (CIC) which is manifested by muscle wasting. glycolysis, tricarboxylic acid (TCA) cycle, and lipid metabolism are central to muscle bioenergy production, which is vulnerable to chemotherapy during cancer treatment. Recent investigations have spotlighted the potential of Shenqi Fuzheng injection (SQ), a Chinese proprietary medicine comprising Radix Codonopsis and Radix Astragali, in alleviating CIC. However, the specific effects of SQ on muscle energy metabolism remains less explored. Purpose and methods: Here, we integrated transcriptomics, spatial metabolomics, gas chromatography-mass spectrometry targeted quantitative analysis, and transmission electron microscopy techniques, combined with Seahorse live -cell metabolic analysis to reveal the changes in genes and pathways related to energy metabolism in the CIC model and SQ's protective effects at molecular and functional levels. Results: Our data showed that chemotherapeutic agents caused glycolysis imbalance, which further leads to metabolic derangements of TCA cycle intermediates. SQ maintained glycolysis balance by facilitating pyruvate fluxing to mitochondria for more efficient bioenergy production, which involved a dual effect on promoting functions of mitochondrial pyruvate dehydrogenase complexes and inhibiting lactate dehydrogenase for lactate production. As a result of the sustained pyruvate level achieved by SQ administration, glycolysis balance was maintained, which further led to the preservation of mitochondrial integrity and function of electron transport chain, thereby, ensuring the normal operation of the TCA cycle and the proper synthesis of adenosine triphosphate (ATP). The above results were further validated using the Seahorse live -cell assay. Conclusion: In conclusion, our study highlights SQ as a promising strategy for CIC management, emphasizing its ability to harmonize the homeostasis of the muscle bioenergetic profile. Beyond its therapeutic implications, this study also offers a novel perspective for the development of innovative treatments in the realm of herbal medicine.
关键词	Shenqi Fuzheng injection Chemotherapy-induce cachexia Bioenergetic profiles Glycolysis TCA cycle lipid metabolism
WOS关键词	CANCER ; OXIDATION ; FATIGUE
DOI	10.1016/j.phymed.2023.155269
收录类别	SCI
语种	英语
资助项目	National Key Research and Devel- opment Program of China[2022YFC3501700] ; Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine[2023YZZ02] ; Key-Area Research and Development Program of Guangdong Province[2020B1111110001] ; Sanming Project of Medicine in Shenzhen[SZZYSM202106004] ; National Engineering Research Center of TCM Standardization Technology[2023-ZYBZH-04]
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:001168026900001
出版者	ELSEVIER GMBH
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.183/handle/2S10ELR8/309932
专题	中国科学院上海药物研究所
通讯作者	Hou, Jinjun; Wu, Wanying
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Capital Med Univ, Sch Tradit Chinese Med, Beijing Key Lab TCM Collateral Dis Theory Res, Beijing 100069, Peoples R China 4.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210029, Peoples R China 5.Livzon Grp Ltd, Limin Pharmaceut Factory, Shaoguan 512028, Peoples R China 6.Guangdong Corp Key Lab High end Liquid Med R&D, Industrializat, Shaoguan 512028, Peoples R China
第一作者单位	中国科学院上海药物研究所
通讯作者单位	中国科学院上海药物研究所
推荐引用方式 GB/T 7714	Li, Wei,Zhang, Zijia,Berik, Entezar,et al. Energy preservation for skeletal muscles: Shenqi Fuzheng injection prevents tissue wasting and restores bioenergetic profiles in a mouse model of chemotherapy-induced cachexia[J]. PHYTOMEDICINE,2024,125:13.
APA	Li, Wei.,Zhang, Zijia.,Berik, Entezar.,Liu, Yawen.,Pei, Wenqiang.,...&Guo, De -an.(2024).Energy preservation for skeletal muscles: Shenqi Fuzheng injection prevents tissue wasting and restores bioenergetic profiles in a mouse model of chemotherapy-induced cachexia.PHYTOMEDICINE,125,13.
MLA	Li, Wei,et al."Energy preservation for skeletal muscles: Shenqi Fuzheng injection prevents tissue wasting and restores bioenergetic profiles in a mouse model of chemotherapy-induced cachexia".PHYTOMEDICINE 125(2024):13.